Multifunctional nanoadditives for the thermodynamic and kinetic stabilization of enzymes

Nanoscale. 2011 Oct 5;3(10):4085-7. doi: 10.1039/c1nr10786k. Epub 2011 Sep 6.

Abstract

Stabilization of enzymes has become a major focus in the quest to improve the activity, sustainability and recyclability of enzymes for their successful integration into both industry and medicine. Here, we describe the kinetic and thermodynamic stabilization of a variety of enzymes in the presence of cationic multifunctional polymeric nanoparticles.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aspergillus oryzae / enzymology
  • Enzymes / chemistry
  • Enzymes / metabolism*
  • Kinetics
  • Light
  • Nanoparticles / chemistry
  • Polymethacrylic Acids / chemistry
  • Protein Denaturation
  • Rhodamines / chemistry
  • Scattering, Radiation
  • Thermodynamics
  • beta-Galactosidase / chemistry
  • beta-Galactosidase / metabolism

Substances

  • Enzymes
  • Polymethacrylic Acids
  • Rhodamines
  • polyglycidyl methacrylate
  • beta-Galactosidase
  • rhodamine B