Effects of impressic acid from Acanthopanax koreanum on NF-κB and PPARγ activities

Arch Pharm Res. 2011 Aug;34(8):1347-51. doi: 10.1007/s12272-011-0815-7. Epub 2011 Sep 11.

Abstract

Impressic acid, 3α,11α-dihydroxylup-20(29)-en-28-oic acid, is a lupane-type triterpenoid isolated from Acanthopanax koreanum, which has been used as a Korean folk medicine for rheumatism, hepatitis, diabetes, and inflammatory disorders. Recently, it was reported that impressic acid has inhibitory effects on the LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells and on the nuclear factor of activated T-cells transcription factor activity. Thus, to investigate whether impressic acid has effects on the inhibition of nuclear factor kappa B (NF-κB) and activation of peroxisome proliferator-activated receptor gamma (PPARγ), luciferase reporter assays were used. The effects on the expression of NF-κB and PPARγ target genes were also examined by reverse transcription-polymerase chain reaction. In this study, impressic acid was found to inhibit tumor necrosis factor (TNF)-α induced NF-κB activity and to up-regulate transcriptional activity of PPARγ.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Marrow Cells / metabolism
  • Cell Survival
  • Chromans / pharmacology
  • Cytokines / antagonists & inhibitors*
  • Cytokines / biosynthesis
  • Dendritic Cells / metabolism
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical
  • Eleutherococcus*
  • Hep G2 Cells
  • Humans
  • Inflammation / drug therapy
  • Lipopolysaccharides / metabolism
  • Luciferases / metabolism
  • NF-kappa B / genetics
  • NF-kappa B / metabolism*
  • PPAR gamma / genetics
  • PPAR gamma / metabolism*
  • Plant Leaves
  • RNA / analysis
  • Rheumatic Diseases / drug therapy
  • Thiazolidinediones / pharmacology
  • Transcription Factors / antagonists & inhibitors
  • Transcription Factors / genetics
  • Transcriptional Activation
  • Triterpenes / chemistry
  • Triterpenes / isolation & purification*
  • Triterpenes / pharmacology*
  • Troglitazone

Substances

  • 3,11-dihydroxylup-20(29)-en-28-oic acid
  • Chromans
  • Cytokines
  • Lipopolysaccharides
  • NF-kappa B
  • PPAR gamma
  • Thiazolidinediones
  • Transcription Factors
  • Triterpenes
  • lupane
  • RNA
  • Luciferases
  • Troglitazone