Enzyme-mimic activity of ferric nano-core residing in ferritin and its biosensing applications

Anal Chem. 2011 Nov 15;83(22):8611-6. doi: 10.1021/ac202049q. Epub 2011 Sep 26.

Abstract

Ferritins are nanoscale globular protein cages encapsulating a ferric core. They widely exist in animals, plants, and microbes, playing indispensable roles in iron homeostasis. Interestingly, our study clearly demonstrates that ferritin has an enzyme-mimic activity derived from its ferric nanocore but not the protein cage. Further study revealed that the mimic-enzyme activity of ferritin is more thermally stable and pH-tolerant compared with horseradish peroxidase. Considering the abundance of ferritin in numerous organisms, this finding may indicate a new role of ferritin in antioxidant and detoxification metabolisms. In addition, as a natural protein-caged nanoparticle with an enzyme-mimic activity, ferritin is readily conjugated with biomolecules to construct nanobiosensors, thus holds promising potential for facile and biocompatible labeling for sensitive and robust bioassays in biomedical applications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biosensing Techniques*
  • Ferritins / chemistry*
  • Ferritins / metabolism
  • Horseradish Peroxidase / chemistry
  • Horseradish Peroxidase / metabolism
  • Hydrogen-Ion Concentration
  • Molecular Mimicry*
  • Nanoparticles / chemistry*
  • Particle Size
  • Surface Properties
  • Temperature

Substances

  • Ferritins
  • Horseradish Peroxidase