Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates

Philippe Lanotte; Chloé Plouzeau; Christophe Burucoa; Carole Grélaud; Sophie Guillot; Nicole Guiso; Fabien Garnier

doi:10.1128/JCM.00335-11

Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates

J Clin Microbiol. 2011 Nov;49(11):3943-6. doi: 10.1128/JCM.00335-11. Epub 2011 Sep 14.

Authors

Philippe Lanotte¹, Chloé Plouzeau, Christophe Burucoa, Carole Grélaud, Sophie Guillot, Nicole Guiso, Fabien Garnier

Affiliation

¹ CHRU de Tours, Service de Bactériologie-Virologie, Hôpital Bretonneau, Tours, France.

Abstract

We evaluated the performances of 4 commercial real-time PCR kits for Bordetella pertussis IS481 sequence detection in nasopharyngeal aspirates by comparison with an in-house real-time PCR assay. Among them, the Simplexa Bordetella pertussis/parapertussis assay (Focus Diagnostics), the SmartCycler Bordetella pertussis/parapertussis assay (Cepheid), and Bordetella R-gene (Argene) present sensitivities over 90%. One kit proved unsuitable for routine clinical use.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
Bordetella pertussis / genetics
Bordetella pertussis / isolation & purification*
Humans
Nasopharynx / microbiology*
Reagent Kits, Diagnostic
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Whooping Cough / diagnosis*
Whooping Cough / microbiology

Substances

Reagent Kits, Diagnostic