Abstract
We attempted to determine the benefits of three-channel multiplex real-time PCR and melting curve analysis not only in detecting and distinguishing between nontuberculous mycobacteria (NTM) and the Mycobacterium tuberculosis complex but also in identifying NTM to the species level.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques / methods*
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Humans
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Multiplex Polymerase Chain Reaction / methods*
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Mycobacterium Infections / diagnosis*
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Mycobacterium Infections / microbiology*
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Mycobacterium tuberculosis / classification
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / isolation & purification*
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Nontuberculous Mycobacteria / classification
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Nontuberculous Mycobacteria / genetics
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Nontuberculous Mycobacteria / isolation & purification*
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Real-Time Polymerase Chain Reaction / methods*
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Transition Temperature