Broad bean and pea by-products as sources of fibre-rich ingredients: potential antioxidant activity measured in vitro

J Sci Food Agric. 2012 Feb;92(3):697-703. doi: 10.1002/jsfa.4633. Epub 2011 Sep 15.

Abstract

Background: By-products generated during the processing of plant food can be considered a promising source of dietary fibre as a functional compound. The dietary fibre composition, soluble sugars and antioxidant activity of the extractable polyphenols of pea and broad bean by-products have been analysed in this study.

Results: Total dietary fibre using AOAC methods plus hydrolysis (broad bean pod: 337.3 g kg⁻¹; pea pod: 472.6 g kg⁻¹) is higher (P < 0.05) in both by-products than with the Englyst method (broad bean pod: 309.7 g kg⁻¹; pea pod: 434.6 g kg⁻¹). The main monomers are uronic acids, glucose, arabinose and galactose in broad bean pods. However, pea pods are very rich in glucose and xylose. The soluble sugars analysed by high-performance liquid chromatography in both by-products have glucose as the most important component, followed by sucrose and fructose. The ferric reducing antioxidant power (broad bean pod: 406.4 µmol Trolox equivalents g⁻¹; pea pod: 25.9 µmol Trolox equivalents g⁻¹) and scavenging effect on 2,2-diphenyl-1-picrylhydrazyl radical (EC₅₀ of broad bean pod: 0.4 mg mL⁻¹; EC₅₀ of pea pod: 16.0 mg mL⁻¹) were also measured.

Conclusions: Broad bean and pea by-products are very rich in dietary fibre, particularly insoluble dietary fibre and their extractable polyphenols demonstrate antioxidant activity. Therefore they might be regarded as functional ingredients.

Keywords: antioxidant activity; broad bean pod; by-products; dietary fibre; pea pod; polyphenols.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / analysis*
  • Antioxidants / chemistry
  • Antioxidants / economics
  • Antioxidants / isolation & purification
  • Dietary Carbohydrates / analysis
  • Dietary Carbohydrates / economics
  • Dietary Carbohydrates / metabolism
  • Dietary Fiber / analysis*
  • Dietary Fiber / economics
  • Dietary Fiber / metabolism
  • Fabaceae / chemistry*
  • Food, Fortified* / economics
  • Glucan 1,4-alpha-Glucosidase / metabolism
  • Hydrolysis
  • Industrial Waste / analysis*
  • Industrial Waste / economics
  • Lignin / analysis
  • Lignin / economics
  • Lignin / isolation & purification
  • Lignin / metabolism
  • Pisum sativum / chemistry*
  • Polyphenols / analysis
  • Polyphenols / economics
  • Polyphenols / isolation & purification
  • Seeds / chemistry*
  • Solubility
  • Spain
  • alpha-Amylases / metabolism

Substances

  • Antioxidants
  • Dietary Carbohydrates
  • Dietary Fiber
  • Industrial Waste
  • Polyphenols
  • Lignin
  • alpha-Amylases
  • termamyl
  • Glucan 1,4-alpha-Glucosidase