Involvement of the XRCC1 Arg399Gln gene polymorphism in the development of cervical carcinoma

Int J Biol Markers. 2011 Oct-Dec;26(4):216-20. doi: 10.5301/JBM.2011.8581.

Abstract

Background: Although infection with the human papillomavirus (HPV) is crucial to the development of cervical cancer, it is not considered a sufficient isolated factor to cause this malignancy. The association of the XRCC1 Arg399Gln (rs25487) polymorphism with cervical cancer has been demonstrated in some populations.

Methods: The XRCC1 Arg399Gln genetic variants were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in patients with advanced cervical cancer (n=189) and controls (n=308).

Results: We observed that patients with advanced cervical cancer having the Gln/Gln or Gln/Arg vs Arg/Arg genotype displayed a 1.726-fold increased risk of cervical cancer (95% confidence interval [CI]=1.158-2.572, p=0.007). The odds ratio (OR) for Gln/Gln vs Gln/Arg or Arg/Arg was 1.742 (95% CI=1.073-2.827; p=0.0236). We also found a significantly higher frequency of the XRCC1 399Gln allele in patients with cancer than in controls, with OR=1.489 (95% CI=1.148-1.930, p=0.0026). The p value of the chi-square test for the trend observed for the XRCC1 Arg399Gln polymorphism was also statistically significant (ptrend=0.002). The statistical power of this study amounted to 78% for the Gln/Gln or Gln/Arg genotypes and 61% for the Gln/Gln genotype.

Conclusion: Although the statistical power of our study did not reach 80%, we found a statistically significant association between the XRCC1 399Gln variant and the incidence of cervical cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Repair
  • DNA-Binding Proteins / genetics*
  • Female
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • Risk Factors
  • Uterine Cervical Neoplasms / genetics*
  • X-ray Repair Cross Complementing Protein 1

Substances

  • DNA-Binding Proteins
  • X-ray Repair Cross Complementing Protein 1
  • XRCC1 protein, human