Determination of plasma cell labelling index with bromodeoxyuridine using a double immunoenzymatic technique

M Ffrench; J P Magaud; P Duhaut; P A Bryon; J J Viala

doi:10.3109/10520299009105605

Determination of plasma cell labelling index with bromodeoxyuridine using a double immunoenzymatic technique

Stain Technol. 1990;65(1):31-6. doi: 10.3109/10520299009105605.

Authors

M Ffrench¹, J P Magaud, P Duhaut, P A Bryon, J J Viala

Affiliation

¹ Analytic Cytology Laboratory, C. Bernard University, E. Herriot Hospital, Lyon, France.

PMID: 2193427
DOI: 10.3109/10520299009105605

Abstract

Myeloma plasma cells were double stained using peroxidase and alkaline phosphatase labelled monoclonal anti-BrdU and anti-intracytoplasmic immunoglobulins. Samples were methanol fixed; DNA was denatured with formamide. The results allowed easy identification of plasma cells, their cytological examination and the calculation of percentage of plasma cells in S phase. Good correlation was found with the labelling index obtained with tritiated thymidine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bromodeoxyuridine*
Cell Cycle*
Humans
Immunoenzyme Techniques*
Paraproteinemias / blood*
Paraproteinemias / pathology
Plasma Cells / pathology*

Substances

Bromodeoxyuridine