Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation

K Hayasaka; M Sato; H Mitani; A Shima

doi:10.1016/0305-0491(90)90387-9

Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation

Comp Biochem Physiol B. 1990;96(2):349-54. doi: 10.1016/0305-0491(90)90387-9.

Authors

K Hayasaka¹, M Sato, H Mitani, A Shima

Affiliation

¹ Laboratory of Radiation Biology, Faculty of Science, University of Tokyo, Japan.

PMID: 2193772
DOI: 10.1016/0305-0491(90)90387-9

Abstract

1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents
Cell Division
Cell Line
Cell Transformation, Neoplastic*
Cells, Cultured
Drug Resistance / genetics
Genes, ras*
Gentamicins
Goldfish
Humans
Mice
Transfection*
Urinary Bladder Neoplasms

Substances

Anti-Bacterial Agents
Gentamicins
antibiotic G 418