A novel cold-inducible gene from Pak-choi (Brassica campestris ssp. chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco

Feng Wang; Xilin Hou; Jun Tang; Zhen Wang; Shuming Wang; Fangling Jiang; Ying Li

doi:10.1007/s11033-011-1245-9

A novel cold-inducible gene from Pak-choi (Brassica campestris ssp. chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco

Mol Biol Rep. 2012 Apr;39(4):4553-64. doi: 10.1007/s11033-011-1245-9. Epub 2011 Sep 22.

Authors

Feng Wang¹, Xilin Hou, Jun Tang, Zhen Wang, Shuming Wang, Fangling Jiang, Ying Li

Affiliation

¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

PMID: 21938429
DOI: 10.1007/s11033-011-1245-9

Abstract

WRKY TFs belong to one of the largest families of transcriptional regulators in plants and form integral parts of signaling webs that modulate many plant processes. BcWRKY46, a cDNA clone encoding a polypeptide of 284 amino acids and exhibited the structural features of group III of WRKY protein family, was isolated from the cold-treated leaves of Pak-choi (Brassica campestris ssp. chinensis Makino, syn. B. rapa ssp. chinensis) using the cDNA-AFLP technique. Expression of this gene was induced quickly and strongly in response to various environmental stresses, including low temperatures, ABA, salt and dehydration. Constitutive expression of BcWRKY46 in tobacco under the control of the CaMV35S promoter reduced the susceptibility of transgenic tobacco to freezing, ABA, salt and dehydration stresses. Our studies suggest that BcWRKY46 plays an important role in responding to ABA and abiotic stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / pharmacology
Amino Acid Sequence
Amplified Fragment Length Polymorphism Analysis
Base Sequence
Brassica / drug effects
Brassica / genetics*
Cold Temperature*
DNA, Complementary / genetics
Dehydration
Gene Expression Profiling
Gene Expression Regulation, Plant* / drug effects
Genes, Plant / genetics*
Germination / drug effects
Mannitol / pharmacology
Molecular Sequence Data
Nicotiana / drug effects
Nicotiana / genetics*
Organ Specificity / drug effects
Organ Specificity / genetics
Phenotype
Phylogeny
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism
Plants, Genetically Modified
Real-Time Polymerase Chain Reaction
Salt Tolerance / drug effects
Salt Tolerance / genetics*
Seeds / drug effects
Seeds / genetics
Seeds / growth & development
Sodium Chloride / pharmacology
Stress, Physiological / drug effects
Stress, Physiological / genetics*

Substances

DNA, Complementary
Plant Proteins
Mannitol
Sodium Chloride
Abscisic Acid

Associated data

GENBANK/DQ402470
GENBANK/HM585284