In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog

J Neurosci Methods. 2012 Jan 15;203(1):136-40. doi: 10.1016/j.jneumeth.2011.09.005. Epub 2011 Sep 14.

Abstract

Accurately locating epileptic foci has great importance in advancing the treatment of epilepsy. In this study, epileptic seizures were first induced by intracortical injection of 4-aminopyridine in rats. A fluorescent deoxyglucose substitute, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG), was then continuously injected via the tail vein. Brain glucose metabolism was subsequently monitored by fluorescence imaging of 2-NBDG. The initial uptake rate of 2-NBDG at the injection site of 4-aminopyridine significantly exceeded that of the control injection site, which indicated local hypermetabolism induced by seizures. Our results show that 2-NBDG can be used for localizing epileptic foci.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 4-Aminopyridine / toxicity
  • 4-Chloro-7-nitrobenzofurazan / analogs & derivatives*
  • Animals
  • Brain / metabolism*
  • Convulsants / toxicity
  • Deoxyglucose / analogs & derivatives*
  • Epilepsy / chemically induced
  • Epilepsy / metabolism*
  • Fluorescent Dyes*
  • Glucose / metabolism*
  • Image Processing, Computer-Assisted
  • Male
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Convulsants
  • Fluorescent Dyes
  • Deoxyglucose
  • 4-Aminopyridine
  • 4-Chloro-7-nitrobenzofurazan
  • Glucose
  • 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose