Building a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae

Methods Enzymol. 2011:500:355-70. doi: 10.1016/B978-0-12-385118-5.00018-9.

Abstract

In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast. The biology of the trehalose cycle is briefly reviewed and discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Computer Simulation
  • Enzyme Assays
  • Gene Expression Regulation, Fungal
  • Glucose-6-Phosphate Isomerase / chemistry
  • Glucose-6-Phosphate Isomerase / physiology
  • Glucosyltransferases / chemistry
  • Glucosyltransferases / physiology
  • Glycolysis
  • Kinetics
  • Metabolic Networks and Pathways
  • Models, Biological*
  • Phosphoglucomutase / chemistry
  • Phosphoglucomutase / physiology
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / physiology
  • Stress, Physiological
  • Systems Biology
  • Trehalose / biosynthesis*
  • UTP-Glucose-1-Phosphate Uridylyltransferase / chemistry
  • UTP-Glucose-1-Phosphate Uridylyltransferase / physiology

Substances

  • Saccharomyces cerevisiae Proteins
  • Trehalose
  • Glucosyltransferases
  • trehalose-6-phosphate synthase
  • UTP-Glucose-1-Phosphate Uridylyltransferase
  • Glucose-6-Phosphate Isomerase
  • Phosphoglucomutase