Catalogue of alleles of gliadin-coding loci in durum wheat (Triticum durum Desf.)

Biochimie. 2012 Feb;94(2):551-7. doi: 10.1016/j.biochi.2011.09.004. Epub 2011 Sep 17.

Abstract

Gliadins are seed storage proteins which are characterized by high intervarietal polymorphism and can be used as genetic markers. As a result of our work, a considerably extended catalogue of allelic variants of gliadin component blocks was compiled for durum wheat; 74 allelic variants for four gliadin-coding loci were identified for the first time. The extended catalogue includes a total of 131 allelic variants: 16 for locus Gli-A1(d), 19 for locus Gli-B1(d), 41 for locus Gli-A2(d), and 55 for locus Gli-B2(d). The electrophoretic pattern of the standard cultivar and a diagram are provided for every block identified. The number of alleles per family is quite small for loci Gli-A1(d) and Gli-B1(d) of durum wheat, as contrasted to loci Gli-A2(d) and Gli-B2(d) that are characterized by large families including many alleles. The presence of large block families determines a higher diversity of durum wheat for loci Gli-A2(d) and Gli-B2(d) as compared to Gli-A1(d) and Gli-B1(d). The catalogue of allelic variants of gliadin component blocks can be used by seed farmers to identify durum wheat cultivars and evaluate their purity; by breeders, to obtain homogenous cultivars and control the initial stages of selection; by gene bank experts, to preserve native varieties and the original biotypic composition of cultivars.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Breeding*
  • Electrophoresis, Polyacrylamide Gel
  • Genetic Loci
  • Genetic Markers
  • Gliadin / genetics*
  • Phylogeography
  • Polymorphism, Genetic*
  • Selection, Genetic
  • Triticum / genetics*

Substances

  • Genetic Markers
  • Gliadin