Abstract
An investigation of a chloroform-soluble extract from the roots of Paeonia rockii ssp. rockii yielded three new noroleanane triterpenoids (1-3) together with 19 known compounds. Their structures were established by analysis of the spectroscopic data. The effects of this chloroform-soluble extract and its major constituents on cell proliferation and apoptosis of a panel of human cancer cell lines (melanoma M-14, colon cancer HT-29, breast cancer MCF-7) were evaluated by the MTT bioassay and propidium iodide staining, respectively, in comparison with normal human embryonic kidney cells (HEK-293). Two of the triterpenoids, betulinic acid (4) and oleanolic acid (5), and the crude extract were cytotoxic and induced apoptosis selectively in the M-14 melanoma cell line. This effect was reversed by the caspase-inhibitor z-VAD-fmk, suggesting that such action is mediated by caspase-3 activation.
MeSH terms
-
Amino Acid Chloromethyl Ketones / pharmacology
-
Antineoplastic Agents, Phytogenic / chemistry
-
Antineoplastic Agents, Phytogenic / isolation & purification*
-
Antineoplastic Agents, Phytogenic / pharmacology
-
Apoptosis / drug effects
-
Betulinic Acid
-
Biphenyl Compounds / pharmacology
-
Caspase Inhibitors
-
Drug Screening Assays, Antitumor
-
HEK293 Cells
-
HT29 Cells
-
Humans
-
Italy
-
Oleanolic Acid / analogs & derivatives
-
Oleanolic Acid / chemistry
-
Oleanolic Acid / isolation & purification*
-
Oleanolic Acid / pharmacology
-
Paeonia / chemistry*
-
Pentacyclic Triterpenes
-
Picrates / pharmacology
-
Plant Roots / chemistry
-
Triterpenes / chemistry
-
Triterpenes / isolation & purification*
-
Triterpenes / pharmacology
Substances
-
23-deoxypaeonenoide A
-
3beta,23-dihydroxy-30-nor-olean-12,20-dien-28-oic acid
-
3beta,4beta,23-trihydroxy-24-nor-olean-12-en-28-oic acid
-
Amino Acid Chloromethyl Ketones
-
Antineoplastic Agents, Phytogenic
-
Biphenyl Compounds
-
Caspase Inhibitors
-
Pentacyclic Triterpenes
-
Picrates
-
Triterpenes
-
benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone
-
Oleanolic Acid
-
1,1-diphenyl-2-picrylhydrazyl
-
Betulinic Acid