Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC

Søren Persson; Joan N Jensen; Katharina E P Olsen

doi:10.1128/JCM.05161-11

Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC

J Clin Microbiol. 2011 Dec;49(12):4299-300. doi: 10.1128/JCM.05161-11. Epub 2011 Oct 5.

Authors

Søren Persson¹, Joan N Jensen, Katharina E P Olsen

Affiliation

¹ Department of Microbiological Diagnostics, The National Reference Laboratory for Enteropathogenic Bacteria, Unit of Gastrointestinal Infections, Statens Serum Institut, Ørestads Boulevard 5, 2300 Copenhagen S, Denmark. [email protected]

Abstract

A multiplex PCR method was developed for the detection of Clostridium difficile toxin genes tcdA, tcdB, ctdA, and cdtB and the major in-frame deletion types (18, 39, and 54 bp) of tcdC. The method has high specificity for PCR ribotype 027 and may identify other C. difficile strains of clinical and epidemiological importance.

Publication types

Evaluation Study

MeSH terms

ADP Ribose Transferases / genetics*
Bacterial Proteins / genetics*
Clostridioides difficile / genetics*
Clostridioides difficile / pathogenicity
Enterotoxins / genetics*
Humans
Multiplex Polymerase Chain Reaction / methods*
Repressor Proteins / genetics*
Sensitivity and Specificity
Sequence Deletion*
Virulence Factors / genetics*

Substances

Bacterial Proteins
Enterotoxins
Repressor Proteins
TcdC protein, Clostridium difficile
Virulence Factors
ADP Ribose Transferases