A triple spin-labeling strategy coupled with DEER analysis to detect DNA modifications and enzymatic repair

Mélanie Flaender; Giuseppe Sicoli; Samia Aci-Seche; Thomas Reignier; Vincent Maurel; Christine Saint-Pierre; Yves Boulard; Serge Gambarelli; Didier Gasparutto

doi:10.1002/cbic.201100550

A triple spin-labeling strategy coupled with DEER analysis to detect DNA modifications and enzymatic repair

Chembiochem. 2011 Nov 25;12(17):2560-3. doi: 10.1002/cbic.201100550. Epub 2011 Oct 7.

Authors

Mélanie Flaender¹, Giuseppe Sicoli, Samia Aci-Seche, Thomas Reignier, Vincent Maurel, Christine Saint-Pierre, Yves Boulard, Serge Gambarelli, Didier Gasparutto

Affiliation

¹ Institut Nanoscience et Cryogénie, Laboratoire de Chimie Inorganique et Biologique, UMR-E n°3 CEA-UJF, CEA-Grenoble, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France.

PMID: 21984076
DOI: 10.1002/cbic.201100550

Abstract

In a spin: Spin-labeled oligonucleotides produced by click chemistry can be studied by EPR, by using a DEER sequence. This was used to test a complex triple-labeling strategy with damaged DNA. Extensive and accurate analysis of DNA structure and enzymatic repair processes were performed after digestion by EndoIV. Modified DNA structures and DNA-protein interactions can now be readily studied.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Click Chemistry
DNA / metabolism*
DNA Cleavage
DNA Damage
Deoxyribonuclease IV (Phage T4-Induced) / metabolism*
Electron Spin Resonance Spectroscopy
Oligonucleotides / chemistry
Spin Labels*

Substances

Oligonucleotides
Spin Labels
DNA
Deoxyribonuclease IV (Phage T4-Induced)