Efficient production of L-lactic acid from xylose by a recombinant Candida utilis strain

Hideyuki Tamakawa; Shigehito Ikushima; Satoshi Yoshida

doi:10.1016/j.jbiosc.2011.09.002

Efficient production of L-lactic acid from xylose by a recombinant Candida utilis strain

J Biosci Bioeng. 2012 Jan;113(1):73-5. doi: 10.1016/j.jbiosc.2011.09.002. Epub 2011 Oct 12.

Authors

Hideyuki Tamakawa¹, Shigehito Ikushima, Satoshi Yoshida

Affiliation

¹ Central Laboratories for Frontier Technology, KIRIN Holdings Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa 236-0004, Japan. [email protected]

PMID: 21996028
DOI: 10.1016/j.jbiosc.2011.09.002

Abstract

Efficient L-lactic acid production from xylose was achieved using a pyruvate decarboxylase-deficient Candida utilis strain expressing an L-lactate dehydrogenase, an NADH-preferring mutated xylose reductase (XR), a xylitol dehydrogenase and a xylulokinase. The recombinant strain showed 53% increased L-lactic acid production compared with the reference strain expressing native XR (NADPH-preferring).

MeSH terms

Aldehyde Reductase / genetics
Aldehyde Reductase / metabolism
Candida / enzymology
Candida / genetics
Candida / metabolism*
Culture Media / chemistry
D-Xylulose Reductase / genetics
D-Xylulose Reductase / metabolism
Fermentation*
Genetic Vectors
Industrial Microbiology
L-Lactate Dehydrogenase / genetics
L-Lactate Dehydrogenase / metabolism*
Lactic Acid / biosynthesis*
Mutagenesis, Site-Directed
Organisms, Genetically Modified / metabolism
Phosphotransferases (Alcohol Group Acceptor) / genetics
Phosphotransferases (Alcohol Group Acceptor) / metabolism
Plasmids
Temperature
Xylose / metabolism*

Substances

Culture Media
Lactic Acid
Xylose
Aldehyde Reductase
L-Lactate Dehydrogenase
D-Xylulose Reductase
Phosphotransferases (Alcohol Group Acceptor)
xylulokinase