Abstract
Fpr3 is the third member of the FKBP (FK506 binding protein) family in yeast. In this study, the fpr3 gene from Saccharomyces cerevisiae was overexpressed and the protein product was purified using different methods. The recombinant Fpr3 fusion protein (rFpr3) was then used as antigen to immunize BALB/c mice for the production of monoclonal antibodies (MAb). Western blot and ELISA results indicated that rFpr3 had specific binding ability to the MAbs, and isotyping results classified the MAb as the subclass IgG1 by antibody. The MAbs obtained in this study will be used as a molecular chaperone to obtain Fpr3 crystals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal, Murine-Derived / biosynthesis*
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Antibody Specificity
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Blotting, Western
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Enzyme-Linked Immunosorbent Assay
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Hybridomas / metabolism
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Immunoglobulin G / biosynthesis*
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Immunophilins / biosynthesis
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Immunophilins / immunology*
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Immunophilins / isolation & purification
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Mice
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Mice, Inbred BALB C
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Molecular Chaperones / biosynthesis*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / immunology*
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Recombinant Fusion Proteins / isolation & purification
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Saccharomyces cerevisiae Proteins / biosynthesis
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Saccharomyces cerevisiae Proteins / immunology*
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Saccharomyces cerevisiae Proteins / isolation & purification
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Titrimetry
Substances
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Antibodies, Monoclonal, Murine-Derived
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Immunoglobulin G
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Molecular Chaperones
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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FPR3 protein, S cerevisiae
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Immunophilins