Validation of 24-hour flucytosine MIC determination by comparison with 48-hour determination by the Clinical and Laboratory Standards Institute M27-A3 broth microdilution reference method

Shawn R Lockhart; Carol B Bolden; Naureen Iqbal; Randall J Kuykendall

doi:10.1128/JCM.05479-11

Validation of 24-hour flucytosine MIC determination by comparison with 48-hour determination by the Clinical and Laboratory Standards Institute M27-A3 broth microdilution reference method

J Clin Microbiol. 2011 Dec;49(12):4322-5. doi: 10.1128/JCM.05479-11. Epub 2011 Oct 19.

Authors

Shawn R Lockhart¹, Carol B Bolden, Naureen Iqbal, Randall J Kuykendall

Affiliation

¹ Mycotic Diseases Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mailstop G-11, Atlanta, GA 30333, USA. [email protected]

Abstract

Flucytosine and itraconazole are the only antifungal agents for which the Clinical Laboratory and Standards Institute recommendations include MIC breakpoint readings at 48 h only. Here we show good essential and categorical agreement between the flucytosine MIC readings at 48 and 24 h for Candida species.

Publication types

Research Support, U.S. Gov't, P.H.S.
Validation Study

MeSH terms

Antifungal Agents / pharmacology*
Candida / drug effects*
Flucytosine / pharmacology*
Humans
Itraconazole / pharmacology
Microbial Sensitivity Tests / methods*
Microbial Sensitivity Tests / standards*
Time Factors

Substances

Antifungal Agents
Itraconazole
Flucytosine