Accumulation and activation of natural killer cells in local intraperitoneal HIV-1/MuLV infection results in early control of virus infected cells

Susanne E Johansson; Hanna Brauner; Jorma Hinkula; Britta Wahren; Louise Berg; Maria H Johansson

doi:10.1016/j.cellimm.2011.09.005

Accumulation and activation of natural killer cells in local intraperitoneal HIV-1/MuLV infection results in early control of virus infected cells

Cell Immunol. 2011;272(1):71-8. doi: 10.1016/j.cellimm.2011.09.005. Epub 2011 Sep 22.

Authors

Susanne E Johansson¹, Hanna Brauner, Jorma Hinkula, Britta Wahren, Louise Berg, Maria H Johansson

Affiliation

¹ Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. [email protected]

PMID: 22019129
DOI: 10.1016/j.cellimm.2011.09.005

Abstract

Natural killer (NK) cells are important effectors in resistance to viral infections. The role of NK cells in the acute response to human immunodeficiency virus 1 (HIV-1) infected cells was investigated in a mouse model based on a HIV-1/murine leukemia virus (MuLV) pseudovirus. Splenocytes infected with HIV-1/MuLV were injected intraperitoneally and local immunologic responses and persistence of infected cells were investigated. In vivo depletion with an anti-NK1.1 antibody showed that NK cells are important in resistance to virus infected cells. Moreover, NK cell frequency in the peritoneal cavity increased in response to infected cells and these NK cells had a more mature phenotype, as determined by CD27 and Mac-1 expression. Interestingly, after injection of HIV-1/MuLV infected cells, but not MuLV infected cells, peritoneal NK cells had an increased cytotoxic activity. In conclusion, NK cells play a role in the early control of HIV-1/MuLV infected cells in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Neutralizing / adverse effects
Antibodies, Neutralizing / pharmacology*
Cytotoxicity, Immunologic / drug effects
Flow Cytometry
HIV Infections / immunology*
HIV Infections / pathology
HIV Infections / virology
HIV-1 / genetics
HIV-1 / immunology*
HIV-1 / metabolism
Humans
Immunity, Innate*
Injections, Intraperitoneal
Killer Cells, Natural* / cytology
Killer Cells, Natural* / drug effects
Killer Cells, Natural* / immunology
Killer Cells, Natural* / metabolism
Killer Cells, Natural* / virology
Leukemia Virus, Murine / genetics
Leukemia Virus, Murine / immunology*
Leukemia Virus, Murine / metabolism
Lymphocyte Activation
Lymphocyte Depletion
Macrophage-1 Antigen / analysis
Macrophage-1 Antigen / biosynthesis
Macrophages / cytology
Macrophages / immunology
Macrophages / virology
Mice
Mice, Transgenic
Monocytes / cytology
Monocytes / immunology
Monocytes / virology
Reassortant Viruses / genetics
Reassortant Viruses / immunology*
Reassortant Viruses / metabolism
Tumor Necrosis Factor Receptor Superfamily, Member 7 / analysis
Tumor Necrosis Factor Receptor Superfamily, Member 7 / biosynthesis
Viral Load / drug effects
Viral Load / immunology

Substances

Antibodies, Neutralizing
Macrophage-1 Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 7