This study analyzed the results of stem cell transplant (SCT) in elderly patients with acute myeloid leukemia (AML) who achieved complete remission (CR) with a gemtuzumab ozogamicin (GO)-containing regimen in order to identify the impact of the drug as a remission induction regimen on the outcome of SCT. Patients who achieved CR with GO proceeded to reduced-intensity conditioning or autologous SCT after one or two cycles of consolidation chemotherapy. With a median follow-up of 53.5 months (n - 17), probabilities of overall survival (OS) and disease-free survival (DFS) at 3 years were 58.2 ± 12.1% and 47.1 ± 12.1%, respectively, and the cumulative incidences of relapse and non-relapse mortality were 35.3 ± 12.1%, and 17.7 ± 9.5%, respectively. Patients with high bone marrow CD33 expression at the time of diagnosis showed significantly higher OS and DFS (70.7%, p = 0.008 and 57.1%, p = 0.008, respectively) than those with low expression. The intensification of post-remission treatment using SCT in elderly patients with AML who achieve CR on GO appears to be an effective and feasible treatment modality.