Yeast one-hybrid assays for gene-centered human gene regulatory network mapping

Nat Methods. 2011 Oct 30;8(12):1050-2. doi: 10.1038/nmeth.1764.

Abstract

Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • DNA / genetics
  • Gene Regulatory Networks / genetics*
  • Genes / genetics*
  • Humans
  • Software
  • Transcription Factors / metabolism
  • Two-Hybrid System Techniques*

Substances

  • Transcription Factors
  • DNA