Abstract
An indigo-producing strain was isolated from activated sludge and identified as Comamonas sp. based on 16S rRNA analysis. It produced indigo at 26.5 mg/l with a conversion of indole to indigo of 47%. Indole at 50 mg/l plus 200 mg naphthalene/l gave 32.2 mg indigo/l with a 58% conversion. A pathway for indigo formation is proposed. This is the first study of indigo biosynthesis by Comamonas sp.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosynthetic Pathways / genetics
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Cluster Analysis
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Comamonas / classification
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Comamonas / genetics
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Comamonas / isolation & purification
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Comamonas / metabolism*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Indigo Carmine
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Indoles / metabolism*
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Molecular Sequence Data
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Phylogeny
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Sewage / microbiology*
Substances
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DNA, Bacterial
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DNA, Ribosomal
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Indoles
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RNA, Ribosomal, 16S
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Sewage
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Indigo Carmine