Abstract
In this study, manassantin A (Man A), an herbal medicine isolated from Saururus chinensis (S. chinensis), markedly inhibited 5-lipoxygenase (5-LO)-dependent leukotriene C(4) (LTC(4)) generation in bone marrow-derived mast cells (BMMCs) in a concentration-dependent manner. To investigate the molecular mechanisms underlying the inhibition of LTC(4) generation by Man A, we assessed the effects of Man A on phosphorylation of cytosolic phospholipase A(2) (cPLA(2)) and mitogen-activated protein kinases (MAPKs). Inhibition of LTC(4) generation by Man A was accompanied by a decrease in cPLA(2) phosphorylation, which occurred via the MAPKs including extracellular signal-regulated protein kinase-1/2 (ERK1/2) as well as p38 and c-Jun N-terminal kinase (JNK) pathways. Taken together, the present study suggests the Man A represents a potential therapeutic approach for the treatment of airway allergic-inflammatory diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Anti-Inflammatory Agents / therapeutic use
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Arachidonate 5-Lipoxygenase / metabolism*
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Bone Marrow
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Dose-Response Relationship, Drug
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Enzyme Activation / drug effects
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Inflammation / drug therapy
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Inflammation / metabolism
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Leukotriene C4 / biosynthesis*
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Lignans / isolation & purification
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Lignans / pharmacology*
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Lignans / therapeutic use
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Male
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Mast Cells / drug effects*
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Mast Cells / metabolism
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Mice
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Mice, Inbred BALB C
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Mitogen-Activated Protein Kinases / antagonists & inhibitors*
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Phospholipases A2 / metabolism
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Phosphorylation
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Phytotherapy
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Saururaceae / chemistry*
Substances
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Anti-Inflammatory Agents
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Lignans
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Plant Extracts
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Leukotriene C4
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manassantin A
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Arachidonate 5-Lipoxygenase
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Mitogen-Activated Protein Kinases
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Phospholipases A2