Abstract
Background:
Human memory CD4+ T cells can be either CD300a/c+ or CD300a/c- and subsequent analyses showed that CD4+ effector memory T (T(EM)) cells are mostly CD300a/c+, whereas CD4+ central memory T (T(CM)) cells have similar frequencies of CD300a/c+ and CD300a/c- cells.
Results:
Extensive phenotypical and functional characterization showed that in both T(CM) and T(EM) cells, the CD300a/c+ subset contained a higher number of TH1 (IFN-γ producing) cells. Alternatively, TH17 (IL-17a producing) cells tend to be CD300a/c-, especially in the T(EM) subset. Further characterization of the IL-17a+ cells showed that cells that produce only this cytokine are mostly CD300a/c-, while cells that produce IL-17a in combination with other cytokines, especially IFN-γ, are mostly CD300a/c+, indicating that the expression of this receptor is associated with cells that produce IFN-γ. Co-ligation of the TCR and CD300a/c in CD4+ T cells inhibited Ca2+ mobilization evoked by TCR ligation alone and modulated IFN-γ production on TH1 polarized cells.
Conclusion:
We conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells and that their ligation is capable of modulating TCR mediated signals.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, CD / genetics
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Antigens, CD / immunology
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Antigens, CD / metabolism*
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Antigens, Surface / genetics
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Antigens, Surface / immunology
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Antigens, Surface / metabolism*
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Calcium Signaling / immunology
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Cell Differentiation / immunology
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Cell Lineage / immunology
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Cells, Cultured
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Gene Expression Regulation / immunology
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Humans
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Immunologic Memory
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Immunophenotyping
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Interferon-gamma / metabolism
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Interleukin-17 / metabolism
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Lymphocyte Activation
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / metabolism*
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Receptor Cross-Talk / immunology
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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Receptors, Antigen, T-Cell, alpha-beta / metabolism
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Receptors, Immunologic / genetics
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Receptors, Immunologic / immunology
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Receptors, Immunologic / metabolism*
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism*
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T-Lymphocyte Subsets / pathology
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Th1 Cells / immunology
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Th1 Cells / metabolism*
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Th1 Cells / pathology
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Th17 Cells / immunology
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Th17 Cells / metabolism*
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Th17 Cells / pathology
Substances
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Antigens, CD
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Antigens, Surface
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CD300A protein, human
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CD300C protein, human
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Interleukin-17
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Membrane Glycoproteins
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Receptors, Antigen, T-Cell, alpha-beta
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Receptors, Immunologic
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Interferon-gamma