Abstract
MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a-/-) and sox9b mutant (sox9b-/-) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Binding Sites
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Cartilage / growth & development*
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Cartilage / metabolism
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Chondrocytes / metabolism
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Chondrogenesis / genetics
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Embryonic Development / genetics
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Gene Expression Regulation, Developmental
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Humans
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In Situ Hybridization
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MicroRNAs / genetics*
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MicroRNAs / metabolism*
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Phylogeny
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Protein Binding
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RNA, Small Interfering / genetics
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SOX9 Transcription Factor / genetics*
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SOX9 Transcription Factor / metabolism*
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Ubiquitin-Protein Ligases / genetics
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Zebrafish / genetics
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Zebrafish / growth & development*
Substances
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MicroRNAs
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Mirn140 microRNA, human
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RNA, Small Interfering
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SOX9 Transcription Factor
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SOX9 protein, human
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WWP2 protein, human
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Ubiquitin-Protein Ligases