This study was designed to explore the effects of low voltage electrical stimulation and delay chilling on the ultrastructure of beef longissimus muscle. The results showed that 44.9% of sarcomeres in the ES samples had contractile zones, and 16.9% of sarcomeres adjacent to contractile zones were stretched in 100 views of electron microscopy. The ultrastructures of 60.9% of sarcomeres changed when the samples were electrically stimulated and then delay-chilled. Contractile bands play an important role in meat tenderization during postmortem aging.