Mass spectrometry-based chemoproteomic approaches

Methods Mol Biol. 2012:803:3-13. doi: 10.1007/978-1-61779-364-6_1.

Abstract

The term "chemical proteomics" refers to a research area at the interface of chemistry, biochemistry, and cell biology that focuses on studying the mechanism of action of bioactive small molecule compounds, which comprises the mapping of their target proteins and their impact on protein expression and posttranslational modifications in target cells or tissues of interest on a proteome-wide level. For this purpose, a large arsenal of approaches has emerged in recent years, many of which employing quantitative mass spectrometry. This review briefly summarizes major experiment types employed in current chemical proteomics research.

Publication types

  • Review

MeSH terms

  • Humans
  • Mass Spectrometry / methods*
  • Pharmaceutical Preparations / metabolism*
  • Proteomics / methods*

Substances

  • Pharmaceutical Preparations