Abstract
Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzyl Alcohols / chemistry
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CHO Cells
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Carbocyanines / chemistry*
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Chromans / chemistry
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Cricetinae
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Cyclooctanes / chemistry
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DNA / chemistry
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Drug Stability
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Fluorescence
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Fluorescent Dyes / chemistry*
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Humans
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Kinetics
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Molecular Dynamics Simulation
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Photobleaching / drug effects
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Receptors, Dopamine D2 / metabolism
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Singlet Oxygen / analysis
Substances
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Benzyl Alcohols
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Carbocyanines
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Chromans
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Cyclooctanes
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Fluorescent Dyes
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Receptors, Dopamine D2
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cyanine dye 5
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Singlet Oxygen
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4-nitrobenzyl alcohol
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DNA
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6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid