Small RNA RyhB as a potential tool used for metabolic engineering in Escherichia coli

Biotechnol Lett. 2012 Mar;34(3):527-31. doi: 10.1007/s10529-011-0794-2. Epub 2011 Nov 15.

Abstract

Small RNA (RyhB) was overexpressed artificially using an arabinose-inducible system in Escherichia coli and resulted in more succinate (7-fold) accumulation, which suggested that RyhB had a strong effect on sdhCDAB genes. Acetate was also increased indicating that RyhB had a comprehensive influence on glucose central metabolism. RyhB might therefore be useful for metabolic engineering of E. coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / metabolism
  • Escherichia coli / genetics*
  • Gene Expression Regulation, Bacterial*
  • Gene Expression*
  • Glucose / metabolism
  • Metabolic Engineering / methods*
  • RNA, Small Untranslated / genetics*
  • RNA, Small Untranslated / metabolism
  • Succinic Acid / metabolism

Substances

  • Acetates
  • RNA, Small Untranslated
  • Succinic Acid
  • Glucose