Abstract
In the present study, we have used plasmid-based RNA interference (RNAi) strategy to downregulate the expression of epidermal growth factor receptor (EGFR) in EGFR wild-type (H292) and mutant (H1975) lung tumor models. The targeted knockdown of EGFR by small hairpin RNA not only inhibited growth of H292 xenograft but also inhibited H1975 lung cancer cell and xenograft, which bore L858R/T790M EGFR and was resistant to EGFR tyrosine kinase inhibitors. These data demonstrated that small hairpin RNA was an effective therapy against mutant EGFR-expressing cancer cells and thus considered to be a promising strategy in the treatment of lung cancers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis
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Carcinoma, Non-Small-Cell Lung / genetics
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Carcinoma, Non-Small-Cell Lung / pathology
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Carcinoma, Non-Small-Cell Lung / therapy*
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Cell Line, Tumor
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Cell Proliferation
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ErbB Receptors / antagonists & inhibitors*
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ErbB Receptors / genetics*
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Fatty Acids, Monounsaturated / administration & dosage
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Female
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Humans
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Liposomes
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Lung Neoplasms / genetics
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Lung Neoplasms / pathology
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Lung Neoplasms / therapy*
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Mice
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Mutation*
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Neoplasm Invasiveness
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Quaternary Ammonium Compounds / administration & dosage
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RNA, Small Interfering / genetics*
Substances
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Fatty Acids, Monounsaturated
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Liposomes
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Quaternary Ammonium Compounds
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RNA, Small Interfering
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ErbB Receptors
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1,2-dioleoyloxy-3-(trimethylammonium)propane