Aromatase (CYP19) gene variants influence ovarian response to standard gonadotrophin stimulation

J Assist Reprod Genet. 2012 Feb;29(2):203-9. doi: 10.1007/s10815-011-9673-y. Epub 2011 Nov 17.

Abstract

Purpose: The association of cytochrome P450 aromatase gene CYP19(TTTA) ( n ) polymorphism with ovarian response to FSH stimulation was explored.

Methods: Three hundred women undergoing medically assisted reproduction and 300 women with at least one spontaneous pregnancy participated in the study. CYP19(TTTA) ( n ) polymorphism was genotyped, while serum hormones were determined. During oocyte retrieval, the follicular size, the follicle and oocyte numbers were recorded.

Results: Six CYP19(TTTA) ( n ) alleles with 7 to 12 repeats were revealed. Women homozygous for long CYP19(TTTA) ( n ) alleles presented with lower serum FSH levels at the third day of the menstrual cycle (p < 0.001) and higher large follicle numbers (p < 0.01), compared to women homozygous for short CYP19(TTTA) ( n ) alleles. The CYP19(TTTA) ( 7 ) allele was associated with higher serum FSH levels (p < 0.003), with lower total follicle (p < 0.02) and large follicle numbers (p < 0.03), while CYP19(TTTA) ( 7 ) allele-carriers presented more frequently with small follicles than CYP19(TTTA) ( 7 ) allele-non carriers (p < 0.01).

Conclusions: CYP19 genetic variants were associated with ovarian reserve and response to standard gonadotrophin stimulation of women undergoing in vitro fertilization.

MeSH terms

  • Adult
  • Aromatase / blood
  • Aromatase / genetics*
  • Estradiol / blood
  • Female
  • Follicle Stimulating Hormone / blood
  • Gene Frequency
  • Genetic Association Studies
  • Gonadotropins / administration & dosage*
  • Homozygote
  • Humans
  • Luteinizing Hormone / blood
  • Microsatellite Repeats / genetics*
  • Oocytes / cytology
  • Ovarian Follicle / cytology
  • Ovary / cytology
  • Ovary / metabolism
  • Polymorphism, Genetic
  • Reproductive Techniques, Assisted

Substances

  • Gonadotropins
  • Estradiol
  • Luteinizing Hormone
  • Follicle Stimulating Hormone
  • Aromatase