Human immunodeficiency virus-1 Rev protein activates hepatitis C virus gene expression by directly targeting the HCV 5'-untranslated region

Jing Qu; Zhenhua Yang; Qi Zhang; Weiyong Liu; Youxing Li; Qiong Ding; Fang Liu; Yingle Liu; Zishu Pan; Biao He; Ying Zhu; Jianguo Wu

doi:10.1016/j.febslet.2011.11.008

Human immunodeficiency virus-1 Rev protein activates hepatitis C virus gene expression by directly targeting the HCV 5'-untranslated region

FEBS Lett. 2011 Dec 15;585(24):4002-9. doi: 10.1016/j.febslet.2011.11.008. Epub 2011 Nov 13.

Authors

Jing Qu¹, Zhenhua Yang, Qi Zhang, Weiyong Liu, Youxing Li, Qiong Ding, Fang Liu, Yingle Liu, Zishu Pan, Biao He, Ying Zhu, Jianguo Wu

Affiliation

¹ State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.

PMID: 22094166
DOI: 10.1016/j.febslet.2011.11.008

Abstract

Coinfection with human immunodeficiency virus-1 (HIV-1) and hepatitis C virus (HCV) accelerates hepatitis C disease progression; however, the mechanism underlying this effect is unknown. Here, we investigated the role of HIV-1 in HCV gene expression and the mechanism involved in this regulation. We discovered that HIV-1 Rev protein activates HCV gene expression. We further revealed that Rev binds to the internal loop of the HCV 5'-untranslated region (5'-UTR) to stimulate HCV IRES-mediated translation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5' Untranslated Regions / genetics*
Arginine
Base Sequence
Cell Line
Gene Expression Regulation, Viral*
HIV-1*
Hepacivirus / genetics*
Molecular Sequence Data
Protein Binding
Protein Biosynthesis / genetics
RNA, Viral / genetics
RNA, Viral / metabolism
Substrate Specificity
Up-Regulation / genetics
rev Gene Products, Human Immunodeficiency Virus / chemistry
rev Gene Products, Human Immunodeficiency Virus / metabolism*

Substances

5' Untranslated Regions
RNA, Viral
rev Gene Products, Human Immunodeficiency Virus
rev protein, Human Immunodeficiency Virus-1
Arginine