Abstract
The display of PHB depolymerase (PhaZ(RpiT1) ) from R. pickettii T1 on the surface of E. coli JM109 cells is realized using OprI of P. aeruginosa as the anchoring motif. The fusion protein is stably expressed and its surface localization is verified by immunofluorescence microscopy. The displayed PhaZ(RpiT1) retains its cleaving ability for soluble substrates as well as its ability to adsorb to the PHB surface, and also remains catalycically active in the degradation of insoluble polyester materials, in spite of the possible suppression of the enzyme movement on the polymer surface. The results demonstrate that PhaZ(RpiT1) -displaying E. coli shows potential for use as a whole-cell biocatalyst for the production of (R)-3-hydroxybutyrate monomers from insoluble PHB materials.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3-Hydroxybutyric Acid / biosynthesis*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biocatalysis
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Carboxylic Ester Hydrolases / genetics
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Carboxylic Ester Hydrolases / metabolism*
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Cloning, Molecular
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Gene Expression
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Hydroxybutyrates / metabolism*
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Kinetics
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Lipoproteins / genetics
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Lipoproteins / metabolism*
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Microscopy, Fluorescence
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Plasmids
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Polyesters / metabolism*
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Pseudomonas aeruginosa / chemistry
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Pseudomonas aeruginosa / genetics
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Ralstonia pickettii / chemistry
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Ralstonia pickettii / genetics
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Substrate Specificity
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Surface Properties
Substances
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Bacterial Proteins
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Hydroxybutyrates
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Lipoproteins
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Polyesters
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Recombinant Fusion Proteins
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outer membrane lipoprotein I, Bacteria
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poly-beta-hydroxybutyrate
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Carboxylic Ester Hydrolases
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poly-beta-hydroxybutyrate depolymerase
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3-Hydroxybutyric Acid