Inhibition of microRNA-29 enhances elastin levels in cells haploinsufficient for elastin and in bioengineered vessels--brief report

Arterioscler Thromb Vasc Biol. 2012 Mar;32(3):756-9. doi: 10.1161/ATVBAHA.111.238113. Epub 2011 Nov 17.

Abstract

Objective: The goal of this study was to determine whether antagonizing microRNA (miR)-29 enhances elastin (ELN) levels in cells and tissues lacking ELN.

Methods and results: miR-29 mimics reduced ELN levels in fibroblasts and smooth muscle cells, whereas miR-29 inhibition increased ELN levels. Antagonism of miR-29 also increased ELN levels in cells from patients haploinsufficient for ELN and in bioengineered human vessels.

Conclusion: miR-29 antagonism may promote increased ELN levels during conditions of ELN deficiencies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aortic Stenosis, Supravalvular / genetics
  • Aortic Stenosis, Supravalvular / metabolism
  • Arteries / metabolism*
  • Blood Vessel Prosthesis*
  • Cells, Cultured
  • Compliance
  • Elastin / deficiency
  • Elastin / genetics
  • Elastin / metabolism*
  • Fibroblasts / metabolism*
  • Haploinsufficiency*
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Muscle, Smooth, Vascular / metabolism*
  • Myocytes, Smooth Muscle / metabolism*
  • RNA Interference
  • Tissue Engineering
  • Transfection
  • Up-Regulation

Substances

  • MIRN29a microRNA, human
  • MicroRNAs
  • Elastin