A probe for the detection of methicillin-resistant Staphylococcus aureus

A C Fluit; A T Box; J Verhoef

doi:10.1007/BF01967216

A probe for the detection of methicillin-resistant Staphylococcus aureus

Eur J Clin Microbiol Infect Dis. 1990 Aug;9(8):605-8. doi: 10.1007/BF01967216.

Authors

A C Fluit¹, A T Box, J Verhoef

Affiliation

¹ Laboratory for Medical Microbiology, University Hospital Utrecht, The Netherlands.

PMID: 2209628
DOI: 10.1007/BF01967216

Abstract

An approximately 300 base pair DNA fragment for use as a probe was isolated from methicillin-resistant Staphylococcus aureus DNA partially digested with Sau3AI. This probe hybridized with 25 methicillin-resistant clinical isolates of Staphylococcus aureus belonging to 18 different phage types, but not with 41 clinical isolates susceptible to methicillin.

MeSH terms

DNA Fingerprinting
DNA Probes*
DNA, Bacterial / genetics*
DNA, Bacterial / isolation & purification
Humans
Methicillin Resistance* / genetics
Nucleic Acid Hybridization
Staphylococcus aureus / drug effects
Staphylococcus aureus / genetics
Staphylococcus aureus / isolation & purification*

Substances

DNA Probes
DNA, Bacterial