Human insulin-receptor gene. Partial sequence and amplification of exons by polymerase chain reaction

Diabetes. 1990 Jan;39(1):123-8. doi: 10.2337/diacare.39.1.123.

Abstract

The partial sequence of the human insulin-receptor (hINSR) gene is presented. Using the gene sequence as a guide, we selected pairs of oligonucleotide primers from sites in the introns that flank each exon. These primers allowed each of the 22 exons of the hINSR gene to be amplified in vitro by the polymerase chain reaction. The sequences of the gene and oligonucleotide primers will facilitate studies of genetic variation in the hINSR gene and thereby increase our understanding of the role of this gene in the development of insulin-resistant states and glucose intolerance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Base Sequence
  • DNA / analysis
  • DNA / genetics
  • Exons
  • Gene Amplification
  • Humans
  • Molecular Sequence Data
  • Nucleotides / analysis
  • Polymerase Chain Reaction
  • Receptor, Insulin / analysis
  • Receptor, Insulin / genetics*

Substances

  • Amino Acids
  • Nucleotides
  • DNA
  • Receptor, Insulin

Associated data

  • GENBANK/M23100
  • GENBANK/M32823
  • GENBANK/M32824
  • GENBANK/M32825
  • GENBANK/M32826
  • GENBANK/M32827
  • GENBANK/M32828
  • GENBANK/M32829
  • GENBANK/M32830
  • GENBANK/M32831