Antigen recognition by antibody C836 through adjustment of V(L)/V(H) packing

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1165-7. doi: 10.1107/S1744309111027746. Epub 2011 Sep 24.

Abstract

C836 is a neutralizing monoclonal antibody to human interleukin IL-13 generated by mouse immunization. The crystal structure of the C836 Fab was determined at 2.5 Å resolution and compared with the IL-13-bound form determined previously. This comparison indicates an induced-fit mechanism of antigen recognition through rigid-body rotation of the V(L) and V(H) domains. The magnitude of this rearrangement is one of the largest observed for antibody-protein interactions.

MeSH terms

  • Animals
  • Antigens / chemistry*
  • Antigens / immunology
  • Crystallography, X-Ray
  • Immunoglobulin Variable Region / chemistry*
  • Immunoglobulin Variable Region / immunology
  • Mice
  • Models, Molecular
  • Protein Structure, Tertiary

Substances

  • Antigens
  • Immunoglobulin Variable Region

Associated data

  • PDB/3L7E