Determination of ABO antigens in fingernails using the APAAP (immunoalkaline phosphatase) technique

Forensic Sci Int. 1990 May-Jun;46(1-2):11-4. doi: 10.1016/0379-0738(90)90122-f.

Abstract

Fingernail specimens with adherent nail-bed were taken from autopsy material with blood groups A, AB, B and O. Frozen 4-5-microns sections were submerged and floated carefully during each working step. Portions of fingernails were contaminated with blood and buccal cells, respectively. Furthermore, fingernail fragments of 8 volunteers were embedded in a biocomponent adhesive according to Grieve and Kotowski (Forensic Sci. Soc., 26 29-34) (1986) and cut by the usual microtome technique. APAAP staining is a proper method for demonstrating blood group antigens in fingernails from groove to margin. Frozen sections as well as smallest specimen embedded in a suitable adhesive are applicable for staining procedures. Using freshly prepared artificial stains, blood group constellations of red blood cells and/or buccal cells adherent on the surface of fingernails may be distinguished from the nail matrix.

MeSH terms

  • ABO Blood-Group System / immunology*
  • Humans
  • Immunoenzyme Techniques
  • Isoantigens / analysis*
  • Nails / immunology*

Substances

  • ABO Blood-Group System
  • Isoantigens