Abstract
Two Klebsiella pneumoniae isolates of the same strain, identified in Poland, produced either TEM-47 or TEM-68, which differed by the Arg275Leu substitution. They harbored a few virulence factors, including an iron-chelating factor and capsule overproduction, suggesting that these factors were sufficient to enhance their nosocomial potency. TEM-68 and TEM-47 had similar enzymatic activities, but TEM-68 was less susceptible to inhibitors than TEM-47. These results confirm the role of the Arg275Leu substitution in the evolution of TEM enzymes.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Substitution
-
Cross Infection / epidemiology
-
Cross Infection / microbiology
-
Cross Infection / transmission*
-
Epidemics*
-
Humans
-
Klebsiella Infections / epidemiology
-
Klebsiella Infections / microbiology
-
Klebsiella Infections / transmission
-
Klebsiella pneumoniae / drug effects
-
Klebsiella pneumoniae / enzymology
-
Klebsiella pneumoniae / genetics
-
Klebsiella pneumoniae / pathogenicity*
-
Microbial Sensitivity Tests
-
Mutation
-
Poland / epidemiology
-
Virulence
-
Virulence Factors / biosynthesis*
-
Virulence Factors / genetics
-
beta-Lactamases / biosynthesis*
-
beta-Lactamases / genetics
Substances
-
Virulence Factors
-
beta-lactamase TEM-47
-
beta-lactamase TEM-68
-
beta-Lactamases