Design of a multinuclear Zn(II) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins

Sho-hei Fujishima; Hiroshi Nonaka; Sho-hei Uchinomiya; Yoshiyuki Alex Kawase; Akio Ojida; Itaru Hamachi

doi:10.1039/c1cc16263b

Design of a multinuclear Zn(II) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins

Chem Commun (Camb). 2012 Jan 14;48(4):594-6. doi: 10.1039/c1cc16263b. Epub 2011 Nov 24.

Authors

Sho-hei Fujishima¹, Hiroshi Nonaka, Sho-hei Uchinomiya, Yoshiyuki Alex Kawase, Akio Ojida, Itaru Hamachi

Affiliation

¹ Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto, 615-8510, Japan.

PMID: 22113378
DOI: 10.1039/c1cc16263b

Abstract

A Zn(II) complex (Zn(II)-Ida) was designed as the new fluorescent probe for His-tag fused proteins. Thanks to the tight binding ability to histidine-rich sequences and bright fluorescence property of the Cy5-appended Zn(II)-Ida probes, selective and clear fluorescent imaging of the His-tag fused G-protein coupled receptors on live cell surfaces was carried out.

MeSH terms

Carbocyanines / chemistry*
Coordination Complexes / chemistry*
Fluorescent Dyes / chemistry*
HEK293 Cells
Histidine / analysis*
Humans
Microscopy, Fluorescence
Oligopeptides / analysis*
Receptors, G-Protein-Coupled / analysis*
Zinc / chemistry*

Substances

Carbocyanines
Coordination Complexes
Fluorescent Dyes
His-His-His-His-His-His
Oligopeptides
Receptors, G-Protein-Coupled
cyanine dye 5
Histidine
Zinc