Abstract
Chemical examination of the methanolic extract from the stem bark of Daphne feddei led to the isolation of three new dicoumarin glucosides (1-3), and eight known coumarins, dicoumarins and dicoumarin glucosides. Their structures were elucidated by extensive analysis of spectral data and comparison with the literature values. All compounds were tested for inhibitory activity against lipopolysaccharide-induced NO production in RAW 264.7 macrophages, and compounds 4 and 5 showed potent inhibitory activity with IC₅₀ values of 0.161 and 0.127 μM, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Coumarins / chemistry
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Coumarins / isolation & purification*
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Coumarins / pharmacology*
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Daphne / chemistry*
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology*
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Glucosides / chemistry
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Glucosides / isolation & purification*
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Glucosides / pharmacology*
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Inhibitory Concentration 50
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Mice
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Molecular Structure
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Nitric Oxide / antagonists & inhibitors
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Nitric Oxide / biosynthesis
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Coumarins
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Drugs, Chinese Herbal
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Glucosides
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Lipopolysaccharides
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bicoumol-7'-O-beta-glucopyranoside
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bicoumol-7-O-beta-glucopyranoside
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Nitric Oxide