Mixed SAMs and MALDI-ToF MS: preparation of N-glycosylamine derivative and thioctic acid methyl ester bearing 1,2-dithiolane groups and detection of enzymatic reaction on Au

Hani Mutlak A Hassan; Beatrice A Maltman

doi:10.1016/j.bioorg.2011.10.004

Mixed SAMs and MALDI-ToF MS: preparation of N-glycosylamine derivative and thioctic acid methyl ester bearing 1,2-dithiolane groups and detection of enzymatic reaction on Au

Bioorg Chem. 2012 Feb;40(1):6-9. doi: 10.1016/j.bioorg.2011.10.004. Epub 2011 Nov 9.

Authors

Hani Mutlak A Hassan¹, Beatrice A Maltman²

Affiliations

¹ Manchester Interdisciplinary Biocentre (MIB), University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. Electronic address: [email protected].
² Manchester Interdisciplinary Biocentre (MIB), University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.

PMID: 22130071
DOI: 10.1016/j.bioorg.2011.10.004

Abstract

Herein, we report an enzymatic galactosylation reaction of β-glucopyranosylamide 4 and thioctic acid methyl ester 5 bearing 1,2-dithiolane groups to form a new system of mixed self-assembled monolayers (SAMs) on gold. Characterization of the enzymatic activity was conveniently achieved by mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylglucosamine / analogs & derivatives*
Acetylglucosamine / chemical synthesis
Acetylglucosamine / chemistry
Amines / chemistry*
Enzymes / metabolism*
Gold / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Surface Properties
Thioctic Acid / analogs & derivatives*
Thioctic Acid / chemical synthesis
Thioctic Acid / chemistry

Substances

1,2-dithiolane
Amines
Enzymes
Thioctic Acid
Gold
Acetylglucosamine

Grants and funding

Biotechnology and Biological Sciences Research Council/United Kingdom