Abstract
The murine co-culture assay is used to generate mature osteoclasts from bone marrow precursors by culturing them with osteoblasts that are stimulated with 1,25-dihydroxy vitamin D(3) and prostaglandin E(2). This assay is used particularly to analyse osteoblast-osteoclast interactions and to determine the cell type affected in knock-out or transgenic mice. This chapter describes also the isolation of bone marrow cells from mice and the methods to purify and replate mature osteoclasts.
MeSH terms
-
Acid Phosphatase / analysis
-
Animals
-
Bone Marrow Cells / cytology*
-
Bone Marrow Cells / metabolism
-
Calcitriol / metabolism
-
Cell Separation / methods
-
Cells, Cultured
-
Coculture Techniques / methods*
-
Dinoprostone / metabolism
-
Isoenzymes / analysis
-
Mice
-
Osteoblasts / cytology*
-
Osteoblasts / metabolism
-
Osteoclasts / cytology*
-
Osteoclasts / metabolism
-
Staining and Labeling / methods
-
Tartrate-Resistant Acid Phosphatase
Substances
-
Isoenzymes
-
Acid Phosphatase
-
Tartrate-Resistant Acid Phosphatase
-
Calcitriol
-
Dinoprostone