Enrichment and detection of peptides from biological systems using designed periodic mesoporous organosilica microspheres

Kun Qian; Wenyi Gu; Pei Yuan; Fang Liu; Yunhua Wang; Michael Monteiro; Chengzhong Yu

doi:10.1002/smll.201101770

Enrichment and detection of peptides from biological systems using designed periodic mesoporous organosilica microspheres

Small. 2012 Jan 23;8(2):231-6. doi: 10.1002/smll.201101770. Epub 2011 Dec 2.

Authors

Kun Qian¹, Wenyi Gu, Pei Yuan, Fang Liu, Yunhua Wang, Michael Monteiro, Chengzhong Yu

Affiliation

¹ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia Fax: (+)61-7-33463973.

PMID: 22135224
DOI: 10.1002/smll.201101770

Abstract

Periodic mesoporous organosilica microspheres (PMOMs) are designed with integrated structural features, including a cubic mesostructure, hydrophobic wall composition, a uniform pore size of ≈3 nm, and a spherical morphology in micrometers, all advantageous for size-selective and highly efficient enrichment of peptides from mixtures. Consequently, PMOMs can be used to capture peptides in a range of complex biological systems.

Keywords: E7; microspheres; organosilica; peptides; size-exclusive.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Magnetic Resonance Spectroscopy
Mice
Microscopy, Electron
Microspheres
Nanopores / ultrastructure
Organosilicon Compounds* / chemistry
Papillomavirus E7 Proteins / isolation & purification
Particle Size
Peptides / isolation & purification*
Porosity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Organosilicon Compounds
Papillomavirus E7 Proteins
Peptides