Colonisation and infection due to Enterobacteriaceae producing plasmid-mediated AmpC β-lactamases

J Infect. 2012 Feb;64(2):176-83. doi: 10.1016/j.jinf.2011.11.016. Epub 2011 Nov 23.

Abstract

Objectives: To investigate the epidemiology and clinical features of infections caused by Enterobacteria producing plasmid-mediated AmpC β-lactamases (pAmpC), which are emerging as a cause of resistance to extended-spectrum cephalosporins.

Methods: A prospective multicentre cohort of patients with infection/colonisation due to pAmpC-producing Enterobacteriaceae was performed in 7 Spanish hospitals from February throughout July 2009. pAmpCs were characterised by PCR and sequencing.

Results: 140 patients were included; organisms isolated were Escherichia coli (n = 100), Proteus mirabilis (n = 20), Klebsiella pneumoniae (n = 17), and others (n = 3). Overall, 90% had a chronic underlying condition. The acquisition was nosocomial in 43%, healthcare-associated in 41% (14% of those were nursing home residents), and community in 16%. Only 5% of patients had no predisposing feature for infection with multidrug-resistant bacteria. Nineteen percent of patients were bacteraemic. Inappropriate empirical therapy was administered to 81% of bacteraemic patients, who had a crude mortality rate of 48%. The most frequent enzyme was CMY-2 (70%, predominantly in E. coli and P. mirabilis) followed by DHA-1 (19%, predominantly in K. pneumoniae).

Conclusion: pAmpC-producing Enterobacteriaceae caused nosocomial, healthcare-associated and community infections mainly in predisposed patients. Invasive infections were associated with high mortality which might be partly related to inappropriate empirical therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cephalosporins / therapeutic use
  • Child
  • Child, Preschool
  • Cohort Studies
  • Cross Infection / microbiology
  • Drug Resistance, Multiple, Bacterial*
  • Enterobacteriaceae / drug effects*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae / pathogenicity*
  • Enterobacteriaceae Infections / epidemiology
  • Enterobacteriaceae Infections / microbiology*
  • Enterobacteriaceae Infections / mortality
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Escherichia coli / pathogenicity
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / pathogenicity
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Plasmids
  • Proteus mirabilis / drug effects
  • Proteus mirabilis / genetics
  • Proteus mirabilis / pathogenicity
  • Spain
  • Young Adult
  • beta-Lactam Resistance / genetics
  • beta-Lactamases / biosynthesis
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • Bacterial Proteins
  • Cephalosporins
  • AmpC beta-lactamases
  • beta-Lactamases