BAG2 is a target of the c-Myc gene and is involved in cellular senescence via the p21(CIP1) pathway

Ju Zhang; Xiaomin Lou; Shangbin Yang; Shun He; Lei Yang; Mei Liu; Hongxia Zhu; Qiang Shan; Siyuan Su; Qimin Zhan; Ningzhi Xu; Siqi Liu

doi:10.1016/j.canlet.2011.11.033

BAG2 is a target of the c-Myc gene and is involved in cellular senescence via the p21(CIP1) pathway

Cancer Lett. 2012 May 1;318(1):34-41. doi: 10.1016/j.canlet.2011.11.033. Epub 2011 Dec 4.

Authors

Ju Zhang¹, Xiaomin Lou, Shangbin Yang, Shun He, Lei Yang, Mei Liu, Hongxia Zhu, Qiang Shan, Siyuan Su, Qimin Zhan, Ningzhi Xu, Siqi Liu

Affiliation

¹ Laboratory of Synthetic Biology, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.

PMID: 22146591
DOI: 10.1016/j.canlet.2011.11.033

Abstract

Suppression of c-Myc is likely to induce cellular senescence in many tumors with unclear mechanisms. A proteomics survey indicated that high levels of BCL2-associated athanogene 2 (BAG2) were found in response to c-Myc repression in TRE293 cells. This observation led to the investigation into the role of BAG2 in c-Myc-induced senescence. The association of the c-Myc/SP1 complex with the BAG2 promoter verified the role of c-Myc/SP1 in regulating BAG2 transcription. Furthermore, high levels of BAG2 were found to induce p21(CIP1)-dependent senescence and subsequent carcinogenetic arrest, suggesting its possible role as an indirect activator of the p21(CIP1) pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Western
Cell Proliferation
Cellular Senescence*
Chromatin Immunoprecipitation
Colonic Neoplasms / genetics
Colonic Neoplasms / metabolism
Colonic Neoplasms / pathology
Cyclin-Dependent Kinase Inhibitor p21 / genetics
Cyclin-Dependent Kinase Inhibitor p21 / metabolism*
Electrophoretic Mobility Shift Assay
Fluorescent Antibody Technique
Humans
Immunoenzyme Techniques
Immunoprecipitation
Luciferases / metabolism
Mice
Mice, Nude
Molecular Chaperones / genetics*
Molecular Chaperones / metabolism
Promoter Regions, Genetic / genetics
Proto-Oncogene Proteins c-myc / antagonists & inhibitors
Proto-Oncogene Proteins c-myc / genetics
Proto-Oncogene Proteins c-myc / metabolism*
RNA, Messenger / genetics
RNA, Small Interfering / genetics
Real-Time Polymerase Chain Reaction
Sp1 Transcription Factor / genetics
Sp1 Transcription Factor / metabolism
Spectrometry, Mass, Electrospray Ionization
Transcription, Genetic*
Tumor Cells, Cultured

Substances

BAG2 protein, human
CDKN1A protein, human
Cyclin-Dependent Kinase Inhibitor p21
Molecular Chaperones
Proto-Oncogene Proteins c-myc
RNA, Messenger
RNA, Small Interfering
Sp1 Transcription Factor
Luciferases