Abstract
The group 2 LIM domain protein, Cysteine-rich intestinal protein 2 (CRIP2) was found to play an important role in esophageal squamous cell carcinoma (ESCC) tumorigenesis. Subcellular fractionation studies show that CRIP2 is expressed in the nucleus. Real-time quantitative PCR shows CRIP2 expression is down-regulated in ESCC tissues and cell lines. Functional studies reveal that CRIP2 reduces colony formation, growth, and invasion abilities. Furthermore, over-expression of CRIP2 induces apoptosis through induction of active caspases 3 and 9 proteins. In conclusion, this study shows CRIP2 plays an important role in the development of ESCC.
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics*
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Adaptor Proteins, Signal Transducing / metabolism*
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Animals
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Apoptosis / genetics*
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology*
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Caspase 3 / metabolism
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Caspase 9 / metabolism
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Cell Growth Processes / genetics
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Cell Line, Tumor
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Cell Nucleus / genetics
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Cell Nucleus / metabolism
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Cell Transformation, Neoplastic / genetics
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Cell Transformation, Neoplastic / metabolism
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Down-Regulation
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Esophageal Neoplasms / genetics
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Esophageal Neoplasms / metabolism
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Esophageal Neoplasms / pathology*
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Female
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Humans
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LIM Domain Proteins / genetics*
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LIM Domain Proteins / metabolism*
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplasm Invasiveness / genetics
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Real-Time Polymerase Chain Reaction / methods
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Up-Regulation
Substances
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Adaptor Proteins, Signal Transducing
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CRIP2 protein, human
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LIM Domain Proteins
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Caspase 3
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Caspase 9