Characterization of two mouse models of metastatic pheochromocytoma using bioluminescence imaging

Cancer Lett. 2012 Mar;316(1):46-52. doi: 10.1016/j.canlet.2011.10.019. Epub 2011 Oct 20.

Abstract

Pheochromocytoma is the most common tumor of the adrenal medulla in adults. The lack of sensitive animal models of pheochromocytoma has hindered the study of this tumor and in vivo evaluation of antitumor agents. In this study we generated two sensitive luciferase models using bioluminescent pheochromocytoma cells: an experimental metastasis model to monitor tumor spreading and a subcutaneous model to monitor tumor growth and spontaneous metastasis. These models offer a platform for sensitive, non-invasive and real-time monitoring of pheochromocytoma primary growth and metastatic burden to follow the course of tumor progression and for testing relevant antitumor treatments in metastatic pheochromocytoma.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adrenal Gland Neoplasms / diagnosis
  • Adrenal Gland Neoplasms / genetics
  • Adrenal Gland Neoplasms / pathology*
  • Adrenal Gland Neoplasms / secondary
  • Animals
  • Carcinogenicity Tests / methods
  • Cell Line, Tumor
  • Disease Models, Animal
  • Disease Progression
  • Early Detection of Cancer / methods
  • Female
  • Luciferases / genetics
  • Luminescent Measurements / methods*
  • Magnetic Resonance Imaging / methods
  • Mice
  • Mice, Nude
  • Neoplasm Metastasis
  • Pheochromocytoma / diagnosis
  • Pheochromocytoma / genetics
  • Pheochromocytoma / pathology*

Substances

  • Luciferases