Autoproteolytic fragments are intermediates in the oligomerization/aggregation of the Parkinson's disease protein alpha-synuclein as revealed by ion mobility mass spectrometry

Chembiochem. 2011 Dec 16;12(18):2740-4. doi: 10.1002/cbic.201100569. Epub 2011 Nov 7.

Abstract

Gas-phase protein separation by ion mobility: With its ability to separate the Parkinson's disease protein α-synuclein and its autoproteolytic products-despite the small concentrations of the latter-ion-mobility MS has enabled the characterization of intermediate fragments in in vitro oligomerization-aggregation. In particular, a possible key fragment, the highly aggregating C-terminal fragment, αSyn(72-140), has been revealed.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopolymers / metabolism*
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Mass Spectrometry / methods*
  • Parkinson Disease / metabolism*
  • Proteolysis
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry
  • alpha-Synuclein / metabolism*

Substances

  • Biopolymers
  • alpha-Synuclein